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The luciferase assays are sensitive and convenient due to the absence of endogenous luciferase activity in most cell types and tissue
and real-time cellular events
This lack of interference occurs because steric hindrance of the protonated tertiary amine in HEPES shields the negative charge of DNA to a lesser extent than less substituted buffers
Can additionally be secured with a screw cap with hole (recommended only for non-pressurised applications)
Sodium dihydrogen phosphate monohydrate Ph. Eur., ACS Size:5kg Powder-freeSodium phosphate monobasic monohydrate